Plasmodesmata: Methods and Protocols (Methods in Molecular Biology) [Hardback]

Description: This fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Plasmodesmata: Methods and Protocols, Second Edition serves as a vital guide for all plant scientists, both novice and expert, especially those studying the intricacies of cell-to-cell communication pathways. Part I: Overview Articles 1. Plasmodesmata Structural Components and Their Role in Signaling and Plant Development Philip Kirk and Yoselin Benitez-Alfonso 2. Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses Caiping Huang and Manfred Heinlein Part II: Structure and Distribution of Plasmodesmata 3. Plasmodesmata Ultrastructure Determination Using Electron Tomography Jules D. Petit, Marie Glavier, Lysiane Brocard, and Emmanuelle Bayer 4. Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata Kamila Godel-Jedrychowska, Tilman Franke, and Ewa Kurczynska 5. Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots Andrea Paterlini and Ilya Belevich 6. Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities Brandon C. Reagan, John R. Dunlap, and Tessa M. Burch-Smith 7. Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy Florence R. Danila 8. Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy Kirsten Knox Part III: Compositional Analysis of Plasmodesmata 9. In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata Caiping Huang, Jerôme Mutterer, and Manfred Heinlein 10. Immunofluorescence Detection of Callose in Plant Tissue Sections Sam Amsbury and Yoselin Benitez-Alfonso 11. Callose Detection and Quantification at Plasmodesmata in Bryophytes Arthur Muller, Tomomichi Fujita, and Yoan Coudert 12. Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis Laetitia Fouillen, Stéphane Claverol, Emmanuelle M.F. Bayer, and Magali S. Grison 13. Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons Verena Kriechbaumer and Stanley W. Botchway 14. Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer Patrick Blümke, Vicky Howe, and Rüdiger Simon 15. Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy Joseph F. McKenna 16. Using Steady-State Fluorescence Anisotropy to Study Protein Clustering Ajeet Chaudhary and Kay Schneitz Part IV: Functional Analysis of Plasmodesmata 17. Quantification of Cell-to-Cell Connectivity Using Particle Bombardment Estee E. Tee, Sebastian Samwald, and Christine Faulkner 18. Investigating Plasmodesmata Function in Arabidopsis thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay Jessica C. Fernandez and Tessa M. Burch-Smith 19. Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay Wilson Horner and Jacob O. Brunkard 20. An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins Frank Machin, Yagmur Hasbioglu, and Friedrich Kragler 21. Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP Min Diao and Shanjin Huang 22. Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes Takumi Tomoi, Yoan Coudert, and Tomomichi Fujita 23. Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata PermeabilityEkaterina A. Lazareva, Anastasia K. Atabekova, Alexander A. Lezzhov, Sergey Y. Morozov, Manfred Heinlein, and Andrey G. Solovyev 24. Analysis of the Distribution of Symplasmic Tracers during Zygotic and Somatic Embryogenesis Justyna Wróbel-Marek, Kamila Godel-Jedrychowska, and Ewa Kurczynska 25. Quantifying Intercellular Movement and Protein Stoichiometry for Computational Modeling Lisa Van den Broeck, Mariah Gobble, and Rosangela Sozzani 26. Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction Dawei Yan 27. A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome RescueMunenori Kitagawa and David Jackson Part V: Other Approaches Contributing to Plasmodesmata Research 28. In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG Eduardo J. Peña and Manfred Heinlein 29. Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM) Michael Fuchs and Jan U. Lohmann 30. More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight Eva E. Deinum 31. Measuring Intercellular Interface Area in Plant Tissues Using Quantitative 3D Image Analysis Gwendolyn V. Davis, Richard S. Smith, and George W. Bassel

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End Time: 2025-01-09T02:52:30.000Z

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